The enzyme substrate method uses coliform producing beta galactosidase to decompose the color source substrate ONPG (Ortho-nitrophenyl-β-D-galactopyranoside) to make the culture medium change yellow, Escherichia coli produces β -glucuronidase to decompose the fluorescent chromatic source substrate MUG（ 4-methyl- umbelliferyl-β-glucuronide）produce fluorescence under 366 nm ultraviolet, The principle is used to determine the quality and quantity of total coliform bacteria, fecal coliform (heat coliform bacteria) and Escherichia coli in water.
When a few non target bacteria in the water sample try to react with ONPG or MUG to interfere with the detection results, the matrix of the special formula in the colitech medium can inhibit the reaction process and control the false positive. At the same time, these special formulations can effectively promote the reaction of the biological enzymes and the fixed substrates of the target bacteria. The incidence of false negative is lower than that of the traditional method. Colitech enzyme substrate test reagent is suitable for GB/T5750.12-2006 “drinking water standard detection method” medium. The combined use of Colitech enzyme substrate reagent and 51 or 97 detection plates can be used to detect target bacteria in 100 ml water samples, the detection range of 51 detection plates is 0 ~ 200MPN, and the detection range of 97 determination disk is 0 ~ 2419MPN, without dilution.
Configuration list
| Serial number | specifications | Specification model | Number |
| Program controlled quantitative sealer | 1 | ||
| 1 | host | / | 1 |
| 2 | power line | / | 1 |
| 3 | 51 hole rubber liner | / | 1 |
| 4 | 97 hole rubber liner | / | 1 |
| 5 | 51 hole MPN table | / | 1 |
| 6 | 97 hole MPN table | / | 1 |
| 7 | Certificate | / | 1 |
| 8 | Instructions | / | 1 |
| 9 | Insurance pipe | / | 2 |
| 10 | Sealing machine tray | / | 1 |
| 51 hole detection plate | |||
| 11 | Quantitative range 0-200MPN/100ml | 100 / box | 1 |
| 97 hole detection plate | |||
| 12 | Quantitative range 0-2419MPN/100ml | 100 / box | 1 |
| Coiltech Enzyme substrate assay reagent | |||
| 13 | Coiltech detection reagent | 200 / box | 1 |
| 100ml scale sampling bottle | |||
| 14 | Sodium thiosulfate | 200 / box | 1 |
| Ultraviolet analyzer with darkroom | |||
| 15 | 366nm,254nm | dual wavelength | 1 |
| Colorimetric plate | |||
| 16 | 97 hole positive colorimetric plate | 1 | |
| 17 | 51hole positive colorimetric plate (two selected) | 1 | |
| Related tools | |||
| Iincubator pipette gun head and gun head box sterilizer etc |
